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The Doppler effect is of fundamental importance in laser spectroscopy. The atoms and molecules in the ensemble under observation perform continuously movements with velocities grouped around one central frequency value which is determined by the ensemble’s temperature. This temperature movement of atoms leads to a spectral broadening of the transitions and so limits the application of many devices and methodologies. For atoms enclosed in cells with centimetre-scale size, the laser beam “sees” the atomic ensemble in the same way—with the same velocity distribution of atoms independently of the propagation direction trough the cell. In this case we say that the velocity distribution of atoms is spatially isotropic.

Read more: Atomic vapour nano-layers—a tool for ultra-high resolution laser spectroscopy

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A new mass spectrometry-based method allows the identification of N-glycosylated protein sites in different tissues in a highly efficient way. It has enabled the identification of 6367 N-glycosylated protein sites.

Read more: New MS method to identify glycosylated proteins